Applications of ion exchange chromatography
Ion exchange chromatography is a process for the separation and purification of cations and anions based on their affinity to the ion exchange resin. Ion exchange chromatography is mostly applicable for the separation and purification of biomolecules such as proteins, enzymes, polypeptides, polynucleotides.
- Significant cations and anions separation are possible by the use of ion-exchange methods. It facilitated the study of environmental speciation. Toxic metal pollutants present in aquatic and environmental can be analyzed by ion-exchange methods.
- The study of aerosols can be carried out on Dionex 2000 instrument with a conductivity detector. It showed the presence of iron, aluminum, zinc, calcium, lead, and nickel. The simultaneous analysis of anions showed the presence of chloride, nitrate, and sulfate by photometric and conductometric detectors.
- It is used for the separation of purification blood components. It is also used to detect different kinds of renal diseases.
- It is used for the determination of sulfur compounds and analysis of inorganic anions in the petrochemical and mining industries.
- To analysis micronutrients in soils, we used ion exchange chromatography methods.
Therefore, ion exchange technique has very wide and interesting applications. It provides a sensitive method of analysis. It separated or purification many ions present on molecules, biomolecules in very low time intervals.